To develop a comprehensive standard operating procedure (SOP) for 3D printing in research labs with very low learning curve

With the introduction of budget-friendly commercialized systems within the last decades [1][2], 3D printing, or additive manufacturing technologies such as fused deposition modeling (FDM), stereolithography (SLA), and selective laser sintering (SLS) have been widely adopted in art, design, medicine, and education, etc [3]. Even though there are still some technical challenges such as material biocompatibility, surface roughness/chemistry, bonding strength, and the adaptation to optical/fluorescence microscopy, it offers means to achieve cost-efficient rapid fabrication for basic research and biological applications. The preaching and training of 3D printing also bring unique challenges as the trainees would come from various backgrounds and age groups, some still yet to get exposed to basic training in engineering/design.